SIF-amide immunoreactivity in the brain of the cockroach Leucophaea maderae.

Veranstaltungsname: 104. Jahrestagung der deutschen zoologischen Gesellschaft e.V.

With antibodies against SIFamide (anti-GYRKPPFNGSIFamide antibody by Dr. Akikazu Yasuda) immunocytochemistry on vibratome sections of the cockroach Leucophaea maderae brain were screened to determine whether SIFamides might be present in the circadian system. The circadian pacemaker which controls locomotor activity rhythms was located via transplantation experiments in the accessory medulla (aMe) with associated pigment-dispersing factor-immunoreactive (PDF-ir) neurons. The PDF-ir pacemakers project to the optic neuropils of the ipsi- and contralateral optic lobes as well as via the anterior and posterior optic commissures to different midbrain targets. Antibodies against SIFamide labeled the anterior fiber network of the aMe, but were not colocalized in somata associated with the aMe. With the exception of the anterior optic commissure all branching sites of PDF-ir circadian pacemaker neurons were also innervated by SIFamide-ir processes, without expressing colocalization. Next to 4 prominent large SIFamide-ir somata, also 5 smaller SIFamide-ir somata per hemisphere were observed in
the pars intercerebralis, while 4-5 immunoreactive somata lie ventrally to the calyx, and about 30 small somata anteriorly to the calyx of the mushroom body. The peduncle and the lobes of the mushroom body were not stained, while the central body expressed very strong SIFamide-immunoreactivity, especially in its upper unit. Also, the optic lobe neuropils and the antennal
lobes expressed a fine web of SIFamide-ir processes. Future MALDI studies will search for SIFamide in the cockroach. [DFG STE531-21]

Zuletzt aktualisiert 2018-05-12 um 16:39